GSE79267   Details

GSE Accession GSE79267
Title Gene expression profiling in pediatric acute lymphoblastic leukemia
Submission Date 3/15/16
Last Update Date 3/16/16
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Experiment Type Expression profiling by high throughput sequencing
Contributor Jessica,,Nordlund; Ann-Christine Syvänen
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Summary Total RNA sequencing of 16 primary ALL patient samples with B-cell precursor (BCP) immunophenotype of different cytogenetic backgrounds: t(12;21) ETV6-RUNX1 (n=4), High Hyperploidy (HeH) (n=4), t(9;22) BCR-ABL1 (n=4), MLL/11q23 (n=4).
Overall Design Total RNA was extracted with the AllPrep DNA/RNA Mini Kit with on-column DNase treatment (QIAGEN). Strand-specific RNA-sequencing libraries were generated from 1 μg total RNA with ScriptSeq v1.2 kits (Epicentre, Madison, WI, USA).; ; Submitter states that raw data are not available (even under controlled access) due to patient privacy concerns per ethics committee review.
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