GSE72792   Details

GSE Accession GSE72792
Title Gene expression analysis of untreated and NT-shRNA transduced kidney proximal tubular epithelial cells (PTECs) at day 6 and day 16 after transduction, as well as SETD2-shRNA transduced PTECs at day 25 after transduction.
Submission Date 9/8/15
Last Update Date 1/11/16
Pubmed ID
Experiment Type Expression profiling by array
Contributor Jun,,Li; Klaas Kok; Anke van den Berg; Rolf,H,Sijmons; Helga Westers; Joost Kluiver; Jan Osinga; Maaike,B,van Werkhoven; Marc,A,Seelen; Debora de Jong; Martijn Terpstra
Contact Name
Contact E-mail
Organization Name
Country
Organism Homo sapiens
Organism ID 9606
Organism Synonym man; human
Summary Primary kidney PTECs gradually became senescesince day 16, but SETD2 depletion prevented PTECs from senescence and maintained their proliferation beyond their limited dividing capacity.; Transcriptional profiling of human PTECs, with comparing of non-senescent PTECs (PTECs-day 6), senescent PTECs (PTECs-day16), and SETD2 depleted PTECs at day 25 (SETD2 KD-PTECs-day 25).
Overall Design Three PTECs of different origins were transduced with shRNA constructs against SETD2 (sh1 or sh2), or with a non-targeting sequence. Untreated and NT-shRNA transduced samples were harvest at day 6 and day 16 respectively, SETD2-KD shRNA transduced PTECs were harvest at day 25.
Platform ID
Timepoint Count 0
Timepoints
Disease
Disease ID