GSE54041   Details

GSE Accession GSE54041
Title Using gene chip technology to screen invasion related molecular targets of esophageal cancer cell lines
Submission Date 1/13/14
Last Update Date 1/15/14
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Experiment Type Expression profiling by array
Contributor Qiqi,,Zhang; Zhiqiang Zhang
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Summary By using gene chip technology, we can screen esophageal cancer invasion associated genes to understand esophageal squamous carcinoma invasion related mechanisms.
Overall Design Using the Transwell invasion chamber technology to establish human esophageal squamous carcinoma Eca109 and Eca109 - T4 cell lines with different invasion ability. Extracting Eca109 and Eca109-T4 total RNA, reverse transcribed into cDNA probes which labeled with Cy5 and Cy3 fluorescence, then it were hybridized with the Affymetrix gene chip. Using the Genechip- Scanner 3000 scan hybridization signal, and using the SAM 3.0 software analysis and process data.
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