GSE46227   Details

GSE Accession GSE46227
Title Developmental equivalence of epiblast stem cells (EpiSCs)
Submission Date 4/19/13
Last Update Date 4/22/13
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Experiment Type Expression profiling by array
Contributor Yoji,,Kojima; Patrick,P,Tam
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Organism Mus musculus
Organism ID 10090
Organism Synonym house mouse; mouse
Summary Epiblast stem cells (EpiSCs) were derived from the epiblast or the ectoderm (epi/ect) of pre-gastrula stage to late-bud stage mouse embryos. To identify if the EpiSCs retain any original stage specific characteristics or which developmental stage of epi/ect they most closely related to, we performed microarray analysis to compare the gene expression profile of multiple EpiSC lines with that of epi/ect of 4 different stages.
Overall Design Eighteen EpiSC lines established from R1-129 embryos of different stages and 1 line (EpiSC9) imported were harvested in triplicate cultured separately for 3 days. The * marks the subline thawn at a different time and harvested but originated from the same embryo. EpiSC9_T is an RNA sample provided by Dr Tesar. ESCs, iPSCs and MEFs were prepared accordingly. For the epiblast samples, in order to avoid the averageing effect by pooling samples, we linearly amplified the RNA starting from a single epiblast of ectoderm.
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