GSE40402   Details

GSE Accession GSE40402
Title Clostridium thermocellum ATCC 27405 Transcriptomic Profiles after Exposure to Ethanol, Furfural and Heat Stress
Submission Date 8/27/12
Last Update Date 1/2/13
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Experiment Type Expression profiling by array
Contributor Charlotte,M,Wilson; Shihui Yang; Courtney,M,Johnson; Miguel Rodriguez; Lezlee Dice; Steven,D,Brown
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Organism Hungateiclostridium thermocellum ATCC 27405
Organism ID 203119
Organism Synonym Clostridium thermocellum DSM 1237; Ruminiclostridium thermocellum ATCC 27405; Clostridium thermocellum ATCC 27405
Summary The thermophilic anaerobe Clostridium thermocellum is a candidate consolidated bioprocessing biocatalyst for the conversion of lignocellulosic biomass into ethanol. The microorganism expresses enzymes for both cellulose solubilization and fermentation to produce lignocellulosic ethanol making it a good candidate for industrial biofuel production. Intolerance to stresses routinely encountered during industrial fermentations may hinder the commercial development of this organism. A recently published study by Yang et al., (2012) characterized the physiological and regulatory response of C. thermocellum to ethanol supplementation. Significant changes in nitrogen metabolism and an accumulation of carbon sources were identified, revealing potential targets for metabolic engineering. In the current study, the response of C. thermocellum to heat and furfural shock were compared with the known effects of ethanol shock. Improved tolerance to these stresses are desirable traits for C. thermocellum and further understanding of the effects that these particular stresses have on the organism are the focus of this work.
Overall Design A forty one array study using total RNA recovered from wild-type cultures of Clostridium thermocellum at different time points of 10, 30, 60, and 120 min post-treatment with 3.95 g.L-1 ethanol, 4 g.L-1 furfural or 68°C treatment compred to that of control without treatment. At least two biological replicates were performed for each treatment and control condition.
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