GSE40143   Details

GSE Accession GSE40143
Title Study of expression changes during RPS4-mediated resistance in Arabidopsis using a temperature-inducible system which mirrors pathogen-infection
Submission Date 8/15/12
Last Update Date 8/8/13
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Experiment Type Expression profiling by array
Contributor Servane,,Blanvillain-Baufumé; Jane Parker; Bruno Huettel; Emiel ver Loren van Theemat
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Summary Innate immune responses of plant cells confer the first line of defence against pathogens. Signals generated by activated receptors are integrated inside the cell and converge on transcriptional programmes in the nucleus. The Arabidopsis Toll-related intracellular receptor RPS4 operates inside nuclei to trigger resistance and defence gene reprogramming through the stress response regulator, EDS1.; In this study, a ChIP-seq approach allowed us to identify RPS4 at the chromatin of several genes, including major defense-marker genes.; In order to correlate the presence of RPS4 at the chromatin with possible transcriptional outputs, we studied the transcriptome of plants upon triggering RPS4 signaling.
Overall Design We used transgenic Arabidopsis plants over-expressing RPS4 in EDS1 WT or eds1-2 mutant backgrounds. In the EDS1 WT background, an immune response can be turned on rapidly and synchronously in leaf cells after a switch from high to moderate temperature.; 35S:RPS4 and 35S:RPS4 eds1-2 plants were grown at 28°C for 3.5 weeks, and subsequently shifted to 19°C. Samples were collected before shift (T0) and 8h after shift, in triplicates.; Related RNA-Seq data are provided in GSE40216, and ChIP-Seq data in GSE40329.
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