GSE30750   Details

GSE Accession GSE30750
Title MicroRNA Expression in Pulmonary Hypertension
Submission Date 7/18/11
Last Update Date 6/10/14
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Experiment Type Non-coding RNA profiling by array
Contributor Revathi,,Rajkumar; Afreen,I,Shariff; Ferhaan Ahmad
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Summary PAH is a life-threatening condition characterized by pulmonary arteriolar remodeling, progressive elevation of pulmonary artery pressure, and right heart failure. Using microarrays, we generated microRNA expression profiles in lung tissue from subjects with PAH, PH associated with IPF, and normal controls. Expression of 23 microRNAs was significantly increased in PAH relative to normal controls and predicted mRNA targets of these microRNAs were integrated with our previously published mRNA expression data from the same subjects. Our analysis implicated involvement of these microRNAs in cellular growth and proliferation, protein ubiquitination, and TGF-β, ERK/MAPK, β-adrenergic, and PDGF signaling.
Overall Design Fresh frozen lung tissue specimens from subjects with PAH (n = 18), subjects with PH associated with IPF (n = 5), and normal control subjects (n = 10) were obtained from the University of Pittsburgh Health Sciences Tissue Bank following approval by the University of Pittsburgh Institutional Review Board. One hundred nanograms of RNA were Cy3 labeled using the microRNA Complete Labeling and Hyb kit (Agilent). Following labeling, samples were hybridized on an 8 x 15K Agilent Human Microarray (V3). Microarrays were then washed and scanned on an Agilent DNA microarray scanner, followed by extraction of microRNA expression data using Agilent Feature Extraction Software.
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