GSE3497   Details

GSE Accession GSE3497
Title Cell division cycle in a human cancer cell line (HeLa)
Submission Date 10/21/05
Last Update Date 3/16/12
Pubmed ID 12058064
Experiment Type Expression profiling by array
Contributor Michael,,Whitfield; Janos Demeter
Contact Name
Contact E-mail
Organization Name
Organism Homo sapiens
Organism ID 9606
Organism Synonym man; human
Summary This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays.; Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.; Keywords: Logical Set
Overall Design Computed
Platform ID
Timepoint Count 47
Timepoints gsm: [0 hr, 2 hr, 4 hr, 6 hr, 3 hr, 8 hr, 5 hr, 7 hr, 9 hr, 11 hr, 13 hr, 15 hr, 17 hr, 10 hr, 12 hr, 14 hr, 16 hr, 19 hr, 21 hr, 23 hr, 18 hr, 25 hr, 27 hr, 29 hr, 31 hr, 33 hr, 35 hr, 37 hr, 20 hr, 22 hr, 24 hr, 26 hr, 39 hr, 41 hr, 28 hr, 43 hr, 30 hr, 45 hr, 32 hr, 34 hr, 36 hr, 38 hr, 40 hr, 42 hr, 1 hr, 44 hr, 46 hr]
Disease ID