GSE116163   Details

GSE Accession GSE116163
Title DNA methylation oscillations at CpG poor enhancers in primed embryonic stem cells [amplicon-Seq]
Submission Date 6/22/18
Last Update Date 6/26/18
Pubmed ID
Experiment Type Methylation profiling by high throughput sequencing
Contributor Steffen,,Rulands; Heather,J,Lee; Stephen,J,Clark; Christof Angermueller; Sebastien,A,Smallwood; Felix Krueger; Gavin Kelsey; Oliver Stegle; Benjamin,D,Simons; Wolf Reik
Contact Name
Contact E-mail
Organization Name
Organism Mus musculus
Organism ID 10090
Organism Synonym house mouse; mouse
Summary Mouse embryonic stem cells (ESCs) primed for differentiation display dynamic heterogeneity characterised by stochastic switching between transcriptional states, and recent advances have highlighted the heterogeneous and dynamic nature of DNA methylation in these. Using single cell sequencing we report global oscillations in ESC DNA methylation that affect particularly CpG poor regions including distal enhancers. These oscillations are dependent on DNA methylation turnover by Dnmt3 and Tet enzymes and influence the probability of transcriptional state switching. Our observations suggest that regulated DNA methylation heterogeneity may contribute to lineage priming in cells poised for differentiation.
Overall Design 2i ES cells transferred into serum media and collected at 48 time points. Control cells were 2i ES cells collected at the same time points (i.e. without changing to serum media)
Platform ID
Timepoint Count 48
Timepoints gse: [Timepoints, 48]
Disease ID