GSE8799   Details

GSE Accession GSE8799
Title Global Control of cell cycle transcription by coupled CDK and network oscillators
Submission Date 2007-08-16
Last Update Date 2012-03-17
Pubmed ID 18463633
Experiment Type Expression profiling by array
Contributor David,A,Orlando; Charles,Y,Lin; Jean,Y,Wang; Steven,B,Haase
Contact Name Steven,B,Haase
Contact E-mail
Organization Name Duke University
Country USA
Organism Saccharomyces cerevisiae;Schizosaccharomyces pombe
Organism ID
Organism Synonym S. cerevisiae;brewer's yeast;baker's yeast;fission yeast
Summary Yeast cell cycle transcription dynamics in two S. cerevisae strains: BF264-15DU (MATa ade1 his2 leu2-3, 112 trp1-1 ura3Dns, bar1) [referred to as wild type] and a mutant of the wild type strain, clb1,2,3,4,5,6 GAL1-CLB1, [referred to as cyclin mutant] that does not express S-phase and mitotic cyclins. Both strains were synchronized by elutriation and released into YEP 2% dextrose/1M sorbitol at 30c. 15 samples were taken at 16 min intervals covering ~2 cycles in wild-type and ~1.5 cycles for the mutants.; A significant fraction of the Saccharomyces cerevisiae genome is transcribed periodically during the cell division cycle, suggesting that properly timed gene expression is important for regulating cell cycle events. Genomic analyses of transcription factor localization and expression dynamics suggest that a network of sequentially expressed transcription factors could control the temporal program of transcription during the cell cycle. However, directed studies interrogating small numbers of genes indicate that their periodic transcription is governed by the activity of cyclin-dependent kinases (CDKs). To determine the extent to which the global cell cycle transcription program is controlled by cyclin/CDK complexes, we compared genome-wide transcription dynamics in wild type budding yeast to mutants that do not express S-phase and mitotic cyclins.; Keywords: cell cycle, strain comparison
Overall Design Cell cycle synchrony/time series experiments. G1 cells collected by elutriation was examined over time for 2 cell cycles. Strains compared: wild type vs cyclin mutants. 15 samples per time course at 16 min resolution. 2 biological replicates per strain.
Platform ID
Timepoint Count 30
Timepoints gsm: [30min_, 46min_, 62min_, 78min_, 94min_, 110min_, 126min_, 142min_, 158min_, 174min_, 190min_, 206min_, 222min_, 238min_, 254min_, 38min_, 54min_, 70min_, 86min_, 102min_, 118min_, 134min_, 150min_, 166min_, 182min_, 198min_, 214min_, 230min_, 246min_, 262min_]
Disease lobular neoplasia;
thrombocytopenia-absent radius syndrome;
congenital disorder;
interstitial lung disease
Disease ID 3010;