GSE81317   Details

GSE Accession GSE81317
Title Disruption of Trim9 function abrogates macrophage motility in vivo
Submission Date 9/28/17
Last Update Date
Pubmed ID 29021367
Experiment Type Expression profiling by array
Contributor Debra,A,Tokarz; Heffelinger,K,Amy; Jima,D,Dereje; Gerlach Jamie; Shah Radhika; Rodriguez-Nunez Ivan; Fletcher,A,Ashley; Nordone,K,Shila; Law J.Mac; Heber Steffen; Yoder,A,Jeffrey
Contact Name Dereje,D.,Jima
Contact E-mail ddjima2014@gmail.com
Organization Name North Carolina State University
Country USA
Organism Danio rerio
Organism ID 5/11/16
Organism Synonym leopard danio;zebra danio;zebra fish;zebrafish
Summary The vertebrate immune response is comprised of multiple molecular and cellular components that interface to provide an adequate defense against pathogens. Although much is known on how individual molecules or cells respond to infection, an understanding of the whole-organism response to pathogen exposure remains unresolved, due to the dynamic complexity of the immune system and its interdependent innate and adaptive functionality. Zebrafish larvae provide a unique model for overcoming this obstacle as larvae can successfully defends themselves from pathogens while lacking a functional adaptive immune system during the first few weeks of life, making it possible to examine exclusively the innate immune response in a whole-organism context. It was hypothesized that the transcriptional response of zebrafish larvae to immune agonists would identify known immune-response genes as well as reveal genes that mediate innate immunity in novel ways. In order to test this hypothesis, zebrafish larvae were exposure to the chemically diverse immune agonists, polyinosine-polycytidylic acid (PolyIC) and synthetic triacylated lipoprotein (Pam3CSK4) and transcriptome analyses completed using microarray analyses. This strategy successfully identified known immune response genes, as well as genes that had not been implicated in immune function, including the E3 ubiquitin ligase, tripartite motif 9 (trim9). Although Trim9 expression has been described as “brain specific”, here we demonstrate elevated levels of trim9 transcripts in macrophages after immune stimulation. As Trim9 has been implicated in axonal migration, we investigated and demonstrate that disruption of Trim9 function impairs macrophage chemotaxis and cellular architecture in vivo. These results demonstrate that Trim9 mediates cellular movement and migration in macrophages as well as neurons.
Overall Design Zebrafish larvae were exposed to the chemically diverse immune agonists, polyinosine-polycytidylic acid (PolyIC) and synthetic triacylated lipoprotein (Pam3CSK4) and transcriptome analyses completed using microarray analyses. RNA were extracted at 4, 8, 12, 24 and 36 time points after exposure to PolyIC and Pam3CSK4. At each timepoint we have a untreated controls.
Platform ID
Timepoint Count 36
Timepoints gse: [Timepoints, 36]
Disease thrombocytopenia-absent radius syndrome;
congenital disorder;
thrombocytopenia
Disease ID DOID:14699;
DOID:759;
DOID:1588