GSE80122   Details

GSE Accession GSE80122
Title Mice carrying a human GLUD2 gene recapitulate aspects of human transcriptome and metabolome development
Submission Date 2016-04-11
Last Update Date 2016-04-27
Pubmed ID 27118840
Experiment Type Expression profiling by high throughput sequencing
Contributor Qian,,Li; Song Guo; Xi Jiang; Jaroslaw Bryk; Ronald Naumann; Wolfgang Enard; Masaru Tomita; Masahiro Sugimoto; Philipp Khaitovich; Svante Pääbo
Contact Name Philipp Khaitovich
Contact E-mail khaitovich@eva.mpg.de
Organization Name CAS-MPG Partner Institute for Computational Biology
Country China
Organism Mus musculus
Organism ID
Organism Synonym mouse;house mouse
Summary Whereas all mammals have one glutamate dehydrogenase gene (GLUD1), humans and apes carry an additional gene (GLUD2), which encodes an enzyme with distinct biochemical properties. We inserted human genomic region containing the GLUD2 gene into mice and analyzed the resulting changes in the transcriptome and metabolome during postnatal brain development. Effects were most pronounced early postnatally and affected predominantly genes involved in neuronal development. Remarkably, the effects in the transgenic mice partially parallel the transcriptome and metabolome differences seen between humans and macaques analyzed. Notably, the introduction of GLUD2 did not affect glutamate levels in mice, consistent with observations in the primates. Instead, the metabolic effects of GLUD2 center on the tricarboxylic acid cycle, suggesting that GLUD2 affects carbon flux during early brain development, possibly stimulating lipid biosynthesis.
Overall Design RNA-sequencing of frontal cortex of GLUD2+/- transgenic mice (two lines: line a and line b) and control littermates
Platform ID
Timepoint Count 0
Timepoints []
Disease
Disease ID