GSE70328   Details

GSE Accession GSE70328
Title caArray_kuppe-00390: Broad: Lesional gene expression profiling in cutaneous T-cell lymphoma reveals natural clusters associated with disease outcome
Submission Date 6/27/15
Last Update Date
Pubmed ID 17638852
Experiment Type Expression profiling by array
Contributor None
Contact Name Mervi Heiskanen
Contact E-mail mervi.heiskanen@nih.gov
Organization Name NCI
Country USA
Organism Homo sapiens
Organism ID 6/26/15
Organism Synonym man;human
Summary Cutaneous T-cell lymphoma (CTCL) is defined by infiltration of activated and malignant T cells in the skin. The clinical manifestations and prognosis in CTCL are highly variable. In this study, we hypothesized that gene expression analysis in lesional skin biopsies can improve understanding of the disease and its management. Based on 63 skin samples, we performed consensus clustering, revealing 3 patient clusters. Of these, 2 clusters tended to differentiate limited CTCL (stages IA and IB) from more extensive CTCL (stages IB and III). Stage IB patients appeared in both clusters, but those in the limited CTCL cluster were more responsive to treatment than those in the more extensive CTCL cluster. The third cluster was enriched in lymphocyte activation genes and was associated with a high proportion of tumor (stage IIB) lesions. Survival analysis revealed significant differences in event-free survival between clusters, with poorest survival seen in the activated lymphocyte cluster. Using supervised analysis, we further characterized genes significantly associated with lower-stage/treatment-responsive CTCL versus higher-stage/treatment-resistant CTCL. We conclude that transcriptional profiling of CTCL skin lesions reveals clinically relevant signatures, correlating with differences in survival and response to treatment. Additional prospective long-term studies to validate and refine these findings appear warranted.
Overall Design kuppe-00390; Assay Type: Gene Expression; Provider: Affymetrix; Array Designs: U133AAofAv2; Organism: Homo sapiens (ncbitax); Tissue Sites: Skin; Material Types: total RNA, synthetic_RNA, organism_part, whole_organism; Disease States: Cutaneous T-cell lymphoma
Platform ID
Timepoint Count 62
Timepoints gsm: [: m1, : m2, : m5, : m7, : m8, : m9, : m10, : m11, : m12, : m13, : m14, : m15, : m16, : m17, : m18, : m19, : m20, : m21, : m22, : m23, : m24, : m25, : m27, : m28, : m29, : m30, : m31, : m33, : m34, : m35, : m36, : m37, : m38, : m40, : m41, : m42, : m43, : m44, : m45, : m46, : m47, : m48, : m49, : m50, : m51, : m52, : m53, : m54, : m55, : m56, : m57, : m58, : m59, : m60, : m61, : m62, : m63, : m64, : m65, : m66, : m67, : t1]
Disease lymphoma
Disease ID DOID:60058