GSE6094   Details

GSE Accession GSE6094
Title Clostridium acetobutylicum ATCC 824 time course with Agilent Arrays
Submission Date 2006-10-20
Last Update Date 2012-03-16
Pubmed ID 18631379
Experiment Type Expression profiling by array
Contributor Nathan,,Cheng; Shawn,W,Jones; Ryan Sillers; Carlos,J,Paredes; Eleftherios,T,Papoutsakis
Contact Name Eleftherios,Terry,Papoutsakis
Contact E-mail papoutsakis@dbi.udel.edu
Organization Name University of Delaware
Country USA
Organism Clostridium acetobutylicum;Clostridium acetobutylicum ATCC 824
Organism ID
Organism Synonym
Summary Using our newly designed C. acetobutylicum arrays we analyzed the mRNA changes during a time course experiment (up to 66 h) paying special attention on the study of the late timepoints.; Keywords: time course
Overall Design Samples were taken from a Bioflow II reactor at 6, 7, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 44, 48, 54, 58, and 66 hrs after bioreactor inoculation. Two slides per timepoint were hybridized on a dye swap configuration. The control mRNA was generated by combining samples taken at 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 44, 48, 54, 58, and 66 hrs after bioreactor inoculation. For timepoints 6, 8, 14, 32, 38, 66 our internal quality control check suggested a larger than normal variation of the intensities in the control channel and a third slide was hybridized. The discarded slides are GSM141189, GSM141203, GSM141222, GSM141229 and GSM141242.
Platform ID
Timepoint Count 26
Timepoints gse: [0, 6,, 7,, 8,, 9,, 10,, 12,, 14,, 16,, 18,, 20,, 22,, 24,, 26,, 28,, 30,, 32,, 34,, 36,, 38,, 40,, 44,, 48,, 54,, 58,, 66 hrs]
Disease
Disease ID