GSE117053   Details

GSE Accession GSE117053
Title Direct observation of CRISPR/Cas9 guide RNAs in single cell transcriptomes
Submission Date 7/12/18
Last Update Date 8/14/18
Pubmed ID
Experiment Type Expression profiling by high throughput sequencing
Contributor Yeo,H,Grace; Juez Oscar; Chen Qing; Banerjee Budhaditya; Chu Lendy; Logister Ive; Gifford,K,David; Sherwood,I,Richard
Contact Name
Contact E-mail
Organization Name
Organism ID
Organism Synonym
Summary We introduce poly-adenine CRISPR gRNA-based single cell RNA-sequencing (pAC-Seq), a method enabling simultaneous observation of mutagenic guide RNAs (gRNAs) and their transcriptional consequences in single cell RNA sequencing (scRNA-seq) data. We have made gRNAs robustly visible in scRNA-seq data while maintaining full activity by modifying them with a hardcoded poly-adenine tract. We apply pAC-Seq to study the transcriptomic effects of non-coding CRISPR/Cas9-induced mutations in a pooled screening format. pAC-Seq is a simple, robust, and broadly applicable method of measuring the global effects of genomic mutation.
Overall Design Pooled screening of transcriptomic effects of CRISPR/Cas9-induced non-coding mutations
Platform ID
Timepoint Count 0
Disease ID